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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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Defense of Dissertation Consult with Graduate Chair and Committee. In the latter case, the student will be sent a Plan of Action template to prepare with their advisor. Step 4: The department will.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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A central finding of this statistical approach to NLP has been that simple methods using words, part-of-speech (POS) sequences (such as whether a word is a noun, verb, or preposition), or simple.

If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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If the template concentration was insufficient for sequencing, we re-amplified the amplicon a second time using only M13REV and M13(-21) primers, and again followed this with gel isolation.

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